Submit Manuscript  

Article Details

Iron Oxide Nanoparticles Synthesized Via Green Tea Extract for Doxorubicin Delivery

[ Vol. 17 , Issue. 4 ]


Lei Nie*, Chenlei Cai, Meng Sun, Fang Zhang, Lingyun Zheng, Qi Peng, Amin Shavandi* and Shoufeng Yang   Pages 646 - 657 ( 12 )


Background: Due to the limitation of conventional cancer treatment using chemotherapy, the nanoparticle therapeutics have shown enhanced efficacy with alleviating side effects.

Objective: The aim of this study was to prepare the superparamagnetic iron oxide nanoparticles (TC- SPION) for doxorubicin (DOX) loading and delivery.

Methods: Here, we reported a simple green strategy to fabricate T-C-SPION using green tea extract and citric acid. Also, the anti-cancer drug, DOX, was used as a model drug to fabricate DOX-loaded nanoparticles.

Results: The formed T-C-SPION nanoparticles were spherical with a diameter of 23.8 ± 0.8 nm, as confirmed by Transmission Electron Microscopy (TEM). Besides, Dynamic Light Scattering (DLS) revealed that the prepared nanoparticles were water-dispersible and stable while stored in water for 6 weeks. The CCK-8 assay showed T-C-SPION to have a good cytocompatibility using different iron concentrations (10 ~ 120 ug/mL). Furthermore, T-C-SPION had a higher DOX encapsulation efficiency (Eencaps), around 43.2 ± 1.8 %, which resulted in a lagged release profile of DOX, compared to other types of iron oxide nanoparticles using green tea or citric acid alone. Next, cell viability assay indicated that T-C-SPION with a higher Eencaps showed superior and sustained cytotoxicity compared to the control group.

Conclusion: The developed iron oxide nanoparticles synthesized by green tea extract and citric acid in this paper could be considered as a potential drug carrier for cancer therapy applications.


Nanoparticles, iron oxide, green tea, citric acid, doxorubicin, drug delivery, cytocompatibility.


Henan Key Laboratory of Tea Plant Biology, College of Life Sciences, Xinyang Normal University, Xinyang 464000, Department of Traditional Chinese Medicine, Hebei General Hospital, Shijiazhuang 050000, Henan Key Laboratory of Tea Plant Biology, College of Life Sciences, Xinyang Normal University, Xinyang 464000, College of Life Science & Technology, Huazhong University of Science and Technology, Wuhan 43007, Analysis & Testing Center, Xinyang Normal University, Xinyang 464000, College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang, Henan 464000, BioMatter-Biomass Transformation Lab (BTL), Universite Libre de Bruxelles, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Department of Mechanical Engineering, Member of Flanders Make, KU Leuven (Catholic University of Leuven), Leuven 3001

Graphical Abstract:

Read Full-Text article