Saumya Srivastava and Anjana Pandey* Pages 81 - 89 ( 9 )
Background: The existing methods of analyte detection by various biochemical processes have certain drawbacks including nonlinearity and detection limits. The nanotechnology has paved a way for the development of new devices i.e., nano-biosensors, by amalgamation of nanoparticles with recognition elements. These nano-biosensors have the capabilities to overcome the drawbacks of conventional methods.
Objectives: Present study was planned to analyze the biosensing potential of indium tin oxide (ITO) electrode modified with gold nanoparticles synthesized using aqueous extract of S. podophyllum.
Methods: In present study, first time rapid green synthesis of gold nanoparticles from leaf extract of Syngonium podophyllum is reported. The synthesized nanoparticles were then characterized physically by UV-Visible spectroscopy, particle size analysis, fluorescent spectroscopy, Fourier transform infrared spectroscopy and transmission electron microscopy (TEM), and HR (High resolution)-TEM.
Results: The particles were observed to be in average size range of 30-70 nm. Furthermore, the ITO electrode was modified with the prepared nanoparticles and analyzed by ellipsometry. The electrochemical characteristics of the modified electrodes were analyzed by cyclic voltammetry.
Conclusion: The results exhibited a higher current gain, thus signifying a higher signal amplification at the electrodes and paves the way for their use in electrochemical nanobiosensors.
Gold nanoparticles, green synthesis, FTIR, TEM, ellipsometry, Cyclic voltammetry.
Department of Biotechnology, MNNIT Allahabad, Prayagraj, Uttar Pradesh 211004, Department of Biotechnology, MNNIT Allahabad, Prayagraj, Uttar Pradesh 211004