Submit Manuscript  

Article Details

Phyto-fabrication of Iron Nanoparticles: Characterization and Antibacterial Capacity

[ Vol. 17 , Issue. 2 ]


Afrah E. Mohammed*, Asma S. Algebaly and Mudawi M. Elobeid   Pages 307 - 314 ( 8 )


Introduction: Fabrication of iron nanoparticles (FeNPs) has recently attracted considerable interest due to the varied applications of FeNPs in remediation technologies.

Objective: The current study aimed to develop a green technology approach to fabricate FeNPs by using extracts from two different plant sources, Azadirachta indica leaf and Calligonum comosum root.

Methods: A mixture of FeCl2 and FeCl3 was used to react with the plant extracts which are considered as reducing and stabilizing agents for the generation of FeNPs in one step. Spectroscopy and microscopy techniques were used for the characterization of the FeNPs.

Results: Immediately after mixing the iron solution and the plant extracts, the color of the solution changed to dark brown indicating conversion of Fe ions to FeNPs. This fabrication of FeNPs was confirmed by Zetasizer, transmission electron microscopy, and scanning electron microscopy. FeNPs fabricated by C. comosum were smaller than those fabricated by A. indica. For both plant sources, FeNPs fabricated using the aqueous extract were smaller than those fabricated with the ethanolic extract. Furthermore, antibacterial ability against two bacterial species was demonstrated.

Conclusion: This study provides evidence that plant extracts can fabricate Fe nanoparticles from Fe ions at room temperature. This technique has potential usage in large-scale production and antibacterial applications, including that it could be recommended for use against antibiotic-resistant bacteria.


Phyto-fabrication, iron oxide nanoparticles, Calligonum comosum, Azadirachta indica, antibacterial, antibioticresistant bacteria.


Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Department of Silviculture, Faculty of Forestry, University of Khartoum, Khartoum North, Shambat 13314

Graphical Abstract:

Read Full-Text article