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Highly Sensitive Detection of CB [7] Based on Fluorescence Resonance Energy Transfer between RhB and Gold Nanoparticles

[ Vol. 16 , Issue. 6 ]

Author(s):

Yan Zeng, Qikun Shi, Xiran Yang, Sobhan Chatterjee, Zaosheng Lv and Feng Liang*   Pages 863 - 869 ( 7 )

Abstract:


Background: Cucurbit[n]uril (CB[n], n=5, 6, 7, 8 and 10)is a type of macrocyclic compounds formed by n glycerol units and 2n methylene. The different sizes of the cavity lead to the different chemical characteristics of CB[n]. Therefore, it is very important to distinguish and detect CB[n].

Methods: At room temperature, Rhodamine B was added to a gold nanoparticles solution. The above mixture was respectively mixed with different concentrations of Cucurbituril[n] or β-cyclodextrin solutions. The fluorescence spectra and ultraviolet-visible spectra of samples were determined.

Results: It was found that 1.2nM gold nanoparticles could quench the fluorescence of 1μM Rhodamine B completely. After adding a certain amount of Cucurbituril[7], the fluorescence intensity of Rhodamine B was restored. Compared with other macrocyclic compounds, such as Cucurbituril[5], Cucurbituril[6] and β-cyclodextrin, we found that this method had unique selectivity for Cucurbituril[ 7]. Under the optimal conditions, the fluorescence recovery efficiency was linearly proportional to the concentration of Cucurbituril[7] in the range of 0.8-8 μg·mL-1. The detection limit was 0.21 μg·mL-1.

Conclusion: The research established an effective and practical FRET-based detection method for CB[7] with RhB as a donor and the gold nanoparticles as the acceptor. The system had unique and extensive selectivity for CB[7].

Keywords:

Cucurbituril [7], FRET, Gold nanoparticles, Rhodamine B, Fluorescence detection, β-cyclodextrin.

Affiliation:

The State Key Laboratory of Refractories and Metallurgy, Coal Conversion and New Carbon Materials Hubei Key Laboratory, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, The State Key Laboratory of Refractories and Metallurgy, Coal Conversion and New Carbon Materials Hubei Key Laboratory, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, The State Key Laboratory of Refractories and Metallurgy, Coal Conversion and New Carbon Materials Hubei Key Laboratory, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, The State Key Laboratory of Refractories and Metallurgy, Coal Conversion and New Carbon Materials Hubei Key Laboratory, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, The State Key Laboratory of Refractories and Metallurgy, Coal Conversion and New Carbon Materials Hubei Key Laboratory, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, The State Key Laboratory of Refractories and Metallurgy, Coal Conversion and New Carbon Materials Hubei Key Laboratory, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081



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