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Culture Media Composition and Reduction Potential Optimization of Mycelia- free Filtrate for the Biosynthesis of Silver Nanoparticles Using the Fungus Tritrichum oryzae W5H

Author(s):

Muhamad O. Al-limoun *, Haitham N. Qaralleh , Khaled M. Khleifat, Mohammed Al-Anber , Amjad A. Al-Tarawneh , Khalid Y. Al-sharafa , Mohammed H. Kailani , Mohammed A. Zaitoun, Suzan A. Matar and Toqa Al-soub   Pages 1 - 13 ( 13 )

Abstract:


Description: A major focus of nanotechnology concerns is the expansion of the optimization of nanomaterials in purity, size and dispersity.

Method: In the current work, a two-step AgNP synthesis process was optimized at the mycelia-DI water suspension and AgNP formation reaction levels.

Results: Biomass filtrate from the fungal strain Tritirachium oryzae W5H was able to reduce silver nitrate into AgNPs after a 72 h reaction, as indicated by the development of an intense brown color and by UV-vis spectra. The biosynthesis ability of AgNPs was markedly better in the presence of a single carbon and nitrogen source in the culture medium compared to multiple sources of carbon and nitrogen. The optimization results of AgNP formation were indifferent between the two steps and were 20 g biomass, 40°C, pH 7.0, 96 h and 1.0 mM AgNO3. The TEM images of the prepared AgNPs illustrated the presence of 7-75 nm, monodispersed and spherical- to ovular-shaped Ag nanoparticles.

Conclusion: The present work highlights the importance of investigating the process parameters by which the reductant mycelia-free filtrate was prepared. In addition, we explored the promising antibacterial action of the prepared AgNPs against bacterial infections.

Keywords:

Culture media, reduction potential of mycelia-free filtrate, AgNPs biosynthesis, optimization, Tritirachium oryzae W5H, characterization, antibacterial activity

Affiliation:

Department of Biological Sciences, Mutah University, Mutah, Karak, 61710, Department of Medical Laboratory Sciences, Mutah University, Mutah, Karak, 61710, Department of Biological Sciences, Mutah University, Mutah, Karak, 61710, Department of Chemistry, Mutah University, Mutah, Karak, 61710, Prince Faisal Center for Dead Sea, Environmental and Energy Research, Mutah University, Mutah, Karak, 61710, Prince Faisal Center for Dead Sea, Environmental and Energy Research, Mutah University, Mutah, Karak, 61710, Department of Chemistry, The University of Jordan, Amman 11942, Pharmaceutical Sciences Department, Yarmouk University, Irbid, Department of Biological Sciences, Faculty of Science, The University of Jordan, Amman 11942, Department of Biological Sciences, Mutah University, Mutah, Karak, 61710



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