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Bioinspired Synthesis of Copper Nanoparticles and its Efficacy on Seed Viability and Seedling Growth in Mungbean (Vigna radiata L.)

Author(s):

Ajinkya S. Jahagirdar, Sudhir Shende, Aniket Gade and Mahendra Rai*   Pages 1 - 7 ( 7 )

Abstract:


Background: Copper is an important micronutrient required for the growth of the plants. It activates enzymes and helps in protein synthesis in plants. Nanoparticles in the size range from 1 to 100 nm possess unique properties, such as high surface area to volume ratio, size dependent capabilities and unique optical properties, and hence, copper nanoparticles (CuNPs) were evaluated for growth promotion of mung bean (Vigna radiata L.).

Objective: The main aim of the study was to synthesize CuNPs using neem extract, and evaluate their activity on viability of seeds and growth of seedlings in V. radiata.

Methods: Here, we synthesized CuNPs by neem (Azadirachta indica) leaf extract, which was treated with copper sulphate and ascorbic acid. The reduction of copper sulphate to CuNPs was confirmed by UV-Visible spectrophotometer, and was further characterized by XRD, FTIR, NTA, and Zeta potential measurement. The efficacy of biogenic CuNPs (size <50 nm) was evaluated on germination and growth promotion of V. radiata seeds. The copper content was confirmed in CuNPs treated plants after analysis by Atomic absorption spectroscopy (AAS).

Results: CuNPs were synthesized by neem (A. indica) leaf extract as a brown precipitation. Preliminary detection was performed by UV-Visible spectrophotometer, which showed peak at 619 nm. Further characterization by X-ray diffraction confirmed the Face Centered Cubic crystal structure. Fourier Transform Infra Red spectroscopy analysis revealed the presence of amino acids as functional groups from leaf extract. Nanoparticle tracking and analysis (NTA) demonstrated average size of 41±21 nm with concentration of 3.3×109 particles/ml. Zeta potential value was found to be -18.2 mV. The growth promotion effect showed the maximum germination recorded at 100 ppm of CuNPs; while copper ions showed adverse effect on root growth. The AAS analysis demonstrated the increased copper content in the CuNPs treated seedlings than that of control.

Conclusion: It is a first report to demonstrate the positive effect of biogenic CuNPs on growth, nutrition and enhanced seed germination, and hence, CuNPs could be used as a nano-fertilizer after further extensive nursery trials.

Keywords:

Nanotechnology, agriculture, biogenic CuNPs, seed germination, FTIR, nano-fertilizer

Affiliation:

Nanobiotechnology Laboratory, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602(MS), Nanobiotechnology Laboratory, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602(MS), Nanobiotechnology Laboratory, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602(MS), Nanobiotechnology Laboratory, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati 444 602(MS)



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