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Cancerous Cell Destruction Using Low Level Ultrasound in the Presence of Gold Nanoparticles: An in vitro Study on 4T1 Cells

[ Vol. 14 , Issue. 4 ]

Author(s):

Ahmad Shanei, Neda Attaran*, Marziyeh Mirzaeiyan, Mohammad Reza Salamat and Hossein Hejazi   Pages 329 - 334 ( 6 )

Abstract:


Background: Ultrasound can destroy target tissue through various mechanisms, such as acoustic cavitation which can be fatal to cells. The existence of nanoparticles in a liquid provides nucleation sites for the cavitation bubbles and this leads to increase in the quantity of bubbles.

Objective: In this study, we investigated the combination effect of gold nanoparticles and therapeutic ultrasound waves on 4T1 cells.

Method: The 4T1 cells were incubated with gold nanoparticles for 24, 48 and 72 h and divided into 4 groups: (1) control, (2) gold nanoparticles, (3) ultrasound waves alone (4) ultrasound waves in the presence of gold nanoparticles. Cell viability assay was used for the detection of cytotoxicity effects.

Results: The results showed a significant decrease in viability of cells which were irradiated with ultrasound waves in the presence of gold nanoparticles.

Conclusion: Acoustic cavitation in the presence of nanoparticles has been presented as a way to increase therapeutic effects on cells.

Keywords:

Cancerous cells destruction, low level ultrasound, gold nanoparticles, in vitro study, acoustic cavitation, nucleation sites, cell viability assay, cytotoxicity effects.

Affiliation:

Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan

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